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RPS6KB1 Antibody

CAT: 0013-GTR18187004Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18187004Size:100 µg
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Description
P70 S6 Kinase antibody
Product Name Alternative
P70S6Kb antibody, Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody, Ribosomal protein S6 kinase beta 2 antibody
UniProt
P23443
Reactivity
Mouse
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal end of human p70S6K protein.
Target
RPS6KB1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000, IP: 5 μg/mg protein sample, WB: 1:1,000
Purity
This affinity-purified antibody is directed against the human p70S6K protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with p70S6K proteins from human, rat and mouse. Reactivity against homologues from other sources is not known.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA, immunoprecipitation and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 70 kDa in size corresponding to p70S6K by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
54041234
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Isotype
IgG

UniProtKB · P23443

Ribosomal protein S6 kinase beta-1

KS6B1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P23443
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
RPS6KB1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
S6K-beta-1, S6K1
EC number
2.7.11.1
Processing
—
Secondary accessions
B2R779, B4DLT4, B4DTG1, E7ESB8, F6UYM1, Q7Z721
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Coding sequence diversity

Alternative initiationAlternative splicing

Biological process

ApoptosisCell cycleTranslation regulation

Ligand

ATP-bindingNucleotide-binding

Cellular component

CytoplasmMembraneMitochondrionMitochondrion outer membraneNucleusSynapseSynaptosome

Molecular function

KinaseSerine/threonine-protein kinaseTransferase

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