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AKT1 Antibody

CAT: 0013-GTR18186997Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18186997Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
AKT (phospho-T308) antibody
Product Name Alternative
Rabbit anti-AKT pT308 Antibody, AKT1 phospho T308, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
UniProt
P31749
Reactivity
Human, Mouse, Rat
Immunogen
Anti-AKT pT308 polyclonal antibody was produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to residues surrounding threonine 308 of human AKT1 protein.
Target
AKT1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Dilution
ELISA: 1:15,000, IHC: 1:200
Purity
AKT phospho T308 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption (s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated human AKT. Minimal reactivity occurs against non-phosphorylated AKT. Reactivity against AKT from other species may occur but has not yet been tested.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
AKT antibody is phospho specific for pT308 and is tested for western blotting, immunohistochemistry, dot blot, and ELISA. This antibody is suitable for Flow Cytometry.
Tested Applications
DOT, ELISA, IHC, WB
NCBI Accession Number
62241011
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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