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Alpha-1-Trypsin Antibody

CAT: 0013-GTR18186992Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18186992Size:25 µL
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Description
Alpha-1-Trypsin antibody
Product Name Alternative
Rabbit anti-alpha-1-antitrypsin antibody, A1A antibody, A1AT, AAT, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, alpha-1-AT antibody, alpha1 PI antibody, alpha1 proteinase inhibitor antibody, Serpin A1, Anti-α-1-antitrypsin
UniProt
P01009
Reactivity
Human
Immunogen
Human Alpha-1-anti-Trypsin
Target
SERPINA1
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0mg/mL
Dilution
ELISA: 1:10,000 - 1:600,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
Purity
Anti-Human ALPHA-1-ANTI-TRYPSIN Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigen resins attached to a solid phase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Human ALPHA-1-ANTI-TRYPSIN Antibody has been tested by LEISA, SDS-PAGE, and western blot. This product is assayed against 1.0 μg of Human Alpha-1-anti-Trypsin in a standard ELISA using Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG and TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 is suggested for this product.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_000286.3
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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