TNFRSF1A Antibody

CAT: 0013-GTR18186983Size: 25 µLDry Ice: NoHazardous: No

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CAT#:0013-GTR18186983Size:25 µL

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Description

TNF p55 Receptor antibody

Product Name Alternative

Rabbit anti-TNFp55 Receptor Antibody, TBP1 antibody, TNFR1 antibody, TNFR55 antibody, TNFRSF1A antibody, Tumor necrosis factor alpha receptor antibody, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, TNF-R1, Tumor necrosis factor receptor type I, TNF-RI, TNFR-I, p55, p60, CD120a, Tumor necrosis factor receptor superfamily member 1A membrane form, Tumor necrosis factor-binding protein 1, TBPI , TNFRSF1A, TNFAR, TNFR1

UniProt

P19438

Reactivity

Human

Immunogen

The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human sTNFRp55 expressed in Chinese Hamster Cells.

Target

TNFRSF1A

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Concentration

1.0 mg/mL

Dilution

ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000

Purity

Anti-Human TNF p55 Receptor Antibody is an IgG preparation of whole rabbit serum purified by Protein G chromatography. This antibody is directed against the extracellular domain of the cell bound human TNFRp55. In general, this antibody also detects primate sTNFRp55. The antibody does not recognize human sTNFRp75. In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the TNFRp55 found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid. This antibody can be used for FACS analysis. Caution should be exhibited as the F (c) domain of the rabbit IgG molecule may interact with cells non-specifically.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-TNF p55 Receptor (sTNFRp55) antibody has been tested for use in ELISA, radioimmunoassays, immunoblotting, flow cytometry and immunoprecipitation. Reactivity in other immunoassays is unknown. This product has been assayed for the ability to immunoprecipitate antigen and a dilution range of 1:400 to 1:800 is suggested. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. This product has been assayed by immunoblot and a working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against soluble (extracellular) sTNFRp55 at a dilution range of 1:2,000 to 1:8,000. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8,000 is suggested for this immunoassay.

Tested Applications

ELISA, IP, WB

NCBI Accession Number

P19438.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P19438

Tumor necrosis factor receptor superfamily member 1A

TNR1A_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P19438
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: TNFRSF1A
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: A8K4X3, B2RDE4, B3KPQ1, B4DQB7, B4E309, B5M0B5, D3DUR1, Q9UCA4

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Disease

AmyloidosisDisease variant

Biological process

ApoptosisHost-virus interaction

Cellular component

Cell membraneGolgi apparatusMembraneSecreted

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Molecular function

Receptor

Domain

RepeatSignalTransmembraneTransmembrane helix

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