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Protein A Antibody

CAT: 0013-GTR18186972Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186972Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Protein A antibody
Product Name Alternative
Chicken anti-Protein A Antibody, chicken anti Protein A, chicken anti PA
Immunogen
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with Protein A [Staphylococcus aureus].
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1 mg/ml
Dilution
ELISA: 1:500 - 1:2,000, WB: 1:500 - 1:2,000
Purity
This Anti-Protein A Antibody is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus] and anti-Chicken Serum. Cross reactivity against Protein A from other sources is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-PROTEIN A (CHICKEN) Antibody has been tested by western blotting and suitable for ELISA. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry, flow Cytometry, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity.
Tested Applications
DOT, ELISA, WB
Host or Source
Gallus
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgY

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