CCL4 Antibody (HRP)

CAT: 0013-GTR18186873Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18186873Size:100 µg

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Description

MIP-1 beta antibody (Peroxidase)

Product Name Alternative

Rabbit anti-MIP-1 beta Antibody Peroxidase Conjugated, rabbit anti-MIP-1b Antibody HRP Conjugated, CCL4, C-C motif chemokine 4, Small-inducible cytokine A4, Macrophage inflammatory protein 1-beta, MIP-1-beta, ACT-2, T-cell activation protein 2, Protein H400, Lymphocyte activation gene 1 protein, LAG-1, HC21, G-26 T-lymphocyte-secreted protein, MIP-1ß

UniProt

P13236

Reactivity

Human

Immunogen

MIP-1 beta Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-1ß protein.

Target

CCL4

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Immunology & Inflammation

Concentration

1.0 mg/mL

Dilution

ELISA: 1:10,000, WB: 1:500

Purity

Human MIP-1 beta Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-9 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Form

Lyophilized

Storage Conditions

Store MIP-1 beta Antibody Peroxidase Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This protein-A purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in size corresponding to human MIP-1ß protein by western blotting in the appropriate cell lysate or extract. Immunology Research

Tested Applications

ELISA, WB

NCBI Accession Number

NP_002975.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P13236

C-C motif chemokine 4

CCL4_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P13236
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CCL4
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: P22617, Q13704, Q3SXL8, Q6FGI8

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

ChemotaxisInflammatory response

Molecular function

Cytokine

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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