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ISG15 Antibody

CAT: 0013-GTR18186871Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186871Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ISG15 antibody
Product Name Alternative
Rabbit anti-ISG15 Antibody, G1P2 antibody, IFI 15 antibody, IFI15 antibody, Interferon alpha inducible protein antibody, Interferon induced 15 kDa protein antibody
UniProt
P05161
Reactivity
Human
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human ISG15 protein.
Target
ISG15
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Infectious Disease & Virology
Concentration
5.0 mg/mL
Dilution
ELISA: 1:2,000 - 1:10,000, WB: 1:200 - 1:1,000
Purity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This purified polyclonal antibody reacts with human ISG15 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates), especially at lower dilutions. An 18.5 kDa band corresponding to human ISG15 is detected. IFNα or IFNß stimulated HeLa cell lysates can be used as a positive control.
Tested Applications
ELISA, WB
NCBI Accession Number
4826774
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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