Gdf15 Antibody

CAT: 0013-GTR18186823Size: 25 µLDry Ice: NoHazardous: No

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CAT#:0013-GTR18186823Size:25 µL

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Description

NAG-1 antibody

Product Name Alternative

Rabbit anti-Nag1 Antibody , NAG-1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor

UniProt

Q9Z0J7

Reactivity

Human, Mouse

Immunogen

This affinity purified antibody was prepared by repeated immunizations with a peptide corresponding to an amino acid sequence near the C-terminal of mouse NAG-1 protein.

Target

Gdf15

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.13 mg/mL

Dilution

ELISA: 1:100,000 - 1:120,000, WB: 1:500-1:2000 for human NAG-1; 1:3000-1:7000 for mouse NAG-1

Purity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with endogenous NAG-1 protein from mouse and human tissues. A BLAST analysis suggests reactivity with NAG-1 from rat based on 100% homology. Partial reactivity is expected against swine, bovine and dog based on 92% homology. Cross-reactivity with NAG-1 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

This affinity purified NAG-1 antibody has been tested by ELISA and western blotting of mouse and human NAG-1 protein. For detection of NAG-1 in mouse serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal) specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of approximately 14 and 28 kDa in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_035949

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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