GDF15 Antibody (Biotin)

CAT: 0013-GTR18186819Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18186819Size:100 µg

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Description

NAG-1 antibody (Biotin)

Product Name Alternative

Rabbit anti-NAG1 antibody biotin conjugation, biotin conjugated rabbit anti-NAG1 antibody, NAG 1, NAG-1, GDF15, MIC1, MIC-1, GDF-15, PLAB, PTGFB, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, Placental bone morphogenetic protein, Prostate differentiation factor

UniProt

Q99988

Reactivity

Human, Mouse

Immunogen

Nag-1 Antibody Biotin Conjugated antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH.

Target

GDF15

Clonality

Polyclonal

Conjugation

Biotin

Concentration

1.0 mg/mL

Dilution

ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000

Purity

Nag-1 Antibody Biotin Conjugated antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with the C-terminus of endogenous NAG-1 protein from human and mouse tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Nag-1 Antibody Biotin Conjugated antibody has been tested by ELISA and western blotting of human and mouse NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of approximately 14 and 28 kDa in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

Q99988.3

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · Q99988

Growth/differentiation factor 15

GDF15_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q99988
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: GDF15
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: GDF-15
EC number: —
Processing: Precursor
Secondary accessions: O14629, P78360, Q9BWA0, Q9NRT0

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Molecular function

CytokineHormone

Disease

Disease variant

Cellular component

Secreted

Domain

Signal

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