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Gata1 Antibody

CAT: 0013-GTR18186806Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18186806Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Gata1 antibody
Product Name Alternative
Rabbit anti-Gata1 antibody, Gata-1, gata 1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, GF-1, NF-E1 DNA-binding protein, Gf-1
UniProt
P17679
Reactivity
Mouse
Immunogen
Anti-Gata1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to mouse Gata1 peptide.
Target
Gata1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Concentration
0.73 mg/mL
Dilution
ELISA: 1:15,000 - 1:75,000, WB: 1:500 - 1:2,000
Purity
Anti-Gata1 is directed against mouse Gata1. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse and rat based on 100% homology for the immunogen sequence and 93% to human. Cross reactivity with Gata1 homologues from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Gata1 Antibody has been tested by ELISA and Western Blot. Expect a band approximately 43 kDa in size corresponding to mouse Gata1 by western blotting in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_032115.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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