ATR Antibody

CAT: 0013-GTR18186737Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18186737Size:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

ATR antibody

Product Name Alternative

Rabbit anti-ATR antibody, Serine/threonine-protein kinase ATR, Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1, ACTR antibody, AIB1 antibody, amplified in breast cancer 1 antibody, CAGH16 antibody, CBP interacting protein antibody, CTG26 antibody, FRP1, FRP-1, FRP 1

UniProt

Q13535

Reactivity

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATR protein.

Target

ATR

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Concentration

1.28 mg/mL

Dilution

ELISA: 1:15,000 - 1:70,000, WB: 1:1,000 - 1:5,000

Purity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human ATR protein. A BLAST analysis was used to suggest cross-reactivity with ATR from human, mouse, rat, monkey, dog, fish and Xenopus sources based on a 100% homology with the immunizing sequence. Cross-reactivity with ATR from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 301 kDa in size corresponding to ATR by western blotting in the appropriate cell lysate or extract. Lysates from HeLa, U2OS, RAW264.7 or HEK293 cells are suggested for western blotting. ATR is ubiquitously expressed, with highest expression levels in the testes. Note that isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.

Tested Applications

ELISA, WB

NCBI Accession Number

157266317

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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