RSL1D1 Antibody

CAT: 0013-GTR18186736Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18186736Size:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

PBK1 antibody

Product Name Alternative

Rabbit anti-PBK1 antibody, PBK-1, PBK 1, CATX-11, CATX11 antibody, RSL1D1, Cellular senescence inhibited gene protein antibody, CSIG antibody, L12 antibody, Ribosomal L1 domain containing protein 1 antibody

UniProt

O76021

Reactivity

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human PBK1.

Target

RSL1D1

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.1 mg/mL

Dilution

ELISA: 1:2,500 - 1:10,000, IF: 1:100, IP: 1:100, WB: 1:500 - 1:2,000

Purity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human PBK1 protein. A BLAST analysis was used to suggest limited cross-reactivity with PBK1 from mouse based on a 75% homology with the immunizing sequence. Cross-reactivity with PBK1 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 55 kDa in size corresponding to PBK1 by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting both over-expressed and endogenous PBK1. For immunofluorescence microscopy, fix cells with methanol.

Tested Applications

ELISA, IF, IP, WB

NCBI Accession Number

62901068

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · O76021

Ribosomal L1 domain-containing protein 1

RL1D1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: O76021
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: RSL1D1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: B4DJ58, D3DUG7, Q2M1T7, Q6PL22, Q8IWS7, Q8WUZ1, Q9HDA9, Q9Y3Z9

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Domain

Coiled coil

Cellular component

Nucleus

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