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MET Antibody

CAT: 0013-GTR18186718Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186718Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Y1356 antibody
Product Name Alternative
Rabbit anti-c-Met pY1349/pY1356 Antibody, rabbit anti-c-Met dual phosphorylated Antibody, HGF receptor antibody, HGF/SF receptor antibody, HGFR antibody, MET antibody, Met proto oncogene tyrosine kinase antibody, SF receptor, Tyrosine-protein kinase Met, Scatter factor receptor, Proto-oncogene c-Met, Hepatocyte growth factor receptor
UniProt
P08581
Reactivity
Human
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1349 and Y1356 of human c-Met protein.
Target
MET
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Concentration
0.96 mg/ml
Dilution
ELISA: 1:93,000, WB: 1μg/mL
Purity
Phospho c-MET Antibody was produced from monospecific antiserum by immunoaffinity chromatography using dual-phospho-peptide coupled to agarose beads followed by cross-adsorption against nonphospho-peptide. Antibody is specific for human c-Met protein phosphorylated at Y1349 and Y1356. A BLAST analysis was used to suggest cross-reactivity with c-Met from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with c-Met from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store Phospho antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 150 kDa in size corresponding to phosphorylated c-Met protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human c-Met pY1349pY1356 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Tested Applications
ELISA, WB
NCBI Accession Number
42741655
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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