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SMAD3 Antibody

CAT: 0013-GTR18186687Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186687Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SMAD3 antibody
Product Name Alternative
Rabbit anti-SMAD3 antibody, SMAD-3, SMAD 3, mothers against decapentaplegic homolog 3 antibody, MAD homolog 3, Mothers against DPP homolog 3, SMAD family member 3, MADH3, MADH 3, JV15-2
UniProt
P84022
Reactivity
Human, Mouse
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of human SMAD3 protein.
Target
SMAD3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Concentration
1.2 mg/ml
Dilution
ELISA: 1:10,000 - 1:40,000, IHC: 1:500 - 1:3,000, WB: 1:500 - 1:3,000
Purity
SMAD3 affinity-purified antibody is directed against human Smad3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat, swine, and chicken sources based on 100% homology for the immunogen sequence. ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated human SMAD3.
Form
Liquid (sterile filtered)
Storage Conditions
Store Anti-SMAD3 at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. This reagent reacts equally to phosphorylated and non-phosphorylated Smad3 by ELISA and western blotting.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
5174513
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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