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Rif1 Antibody

CAT: 0013-GTR18186664Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186664Size:100 µg
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Description
Rif1 antibody
Product Name Alternative
Rabbit anti-Rif1 antibody, Rif-1, Rif 1, Telomere-associated protein RIF1, Rap1 interacting factor 1 antibody, Rap1 interacting factor 1 homolog antibody, mRif1, Rap-1
UniProt
Q6PR54
Reactivity
Mouse
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 2375-2419 of Mouse Rif1.
Target
Rif1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Concentration
1.0mg/mL
Dilution
ELISA: 1:5,000 - 1:40,000, WB: 1:500 - 1:3,000
Purity
This affinity purified antibody is directed against mouse Rif1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Expect cross reactivity with Rif1 from human, chimpanzee, rat and dog as only a single amino acid residue change (92% homology) is found for the immunogen sequence. Cross reactivity with Rif1 homologues from other sources has not been determined. Databases confirm that the immunogen sequence represents the C-terminal end of mouse Rif1 (534 aa 58.1 kDa), mouse telomere-associated protein RIF1 (2418 aa 265.7 kDa) and mouse Rap1-interacting factor 1 (2426 267.1 kDa) .
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 265 kDa in size corresponding to Rif1 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
68565756
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · Q6PR54

Telomere-associated protein RIF1

RIF1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q6PR54
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Rif1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q6T8D9, Q7TPD8, Q8BTU2, Q8C408, Q9CS77
Protein keywords

Coding sequence diversity

Alternative splicing

Biological process

Cell cycleDNA damageDNA repair

Cellular component

ChromosomeCytoplasmCytoskeletonNucleusTelomere

PTM

Phosphoprotein

Technical term

Reference proteome

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