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SLIT2 Antibody

CAT: 0013-GTR18186647Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186647Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SLIT-2 antibody
Product Name Alternative
Rabbit anti-SLIT-2 antibody, SLIT2, SLIT 2, Slit homolog 2 protein, SLIL3, SLIL-3 antibody
UniProt
O94813
Reactivity
Gallus, Human
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 475-500 of Human SLIT-2 protein.
Target
SLIT2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Concentration
1.11 mg/mL
Dilution
ELISA: 1:5,000 - 1:50,000, IHC: 2ug/ml, WB: 1:500 - 1:2,000
Purity
This affinity purified antibody is directed against human SLIT-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-2 from Tetraodon nigroviridis , as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST) . The core 13 amino acids of the immunogen are also 100% homologous to SLIT-2 from mouse, rat, bovine and Xenopus laevis. Cross reactivity with SLIT-2 homologues from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~ 165 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. For western blotting block the membrane with goat serum. Do not block with non-fat dry milk.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
4759146
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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