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SPRY4 Antibody

CAT: 0013-GTR18186644Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186644Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Sprouty-4 antibody
Product Name Alternative
Rabbit anti-Sprouty4 antibody, Rabbit anti-Sprouty-4 antibody, Sprouty 4, SPRY4, SPRY 4, Spry-4, Protein sprouty homolog 4
UniProt
Q9C004
Reactivity
Human, Mouse
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 275-299 of Human Sprouty-4 protein.
Target
SPRY4
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Concentration
1.1 mg/mL
Dilution
ELISA: 1:3,000 - 1:12,000, IHC: 1 μg/ml to 10 μg/ml, WB: 1:100 - 1:1,000
Purity
This affinity purified antibody is directed against human Sprouty-4 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity is expected with Sprouty-4.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band (maybe a doublet) ~35 kDa in size corresponding to Sprouty-4 by western blotting in the appropriate cell lysate or extract. Some non-specific banding may be observed using this antibody.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_001120968.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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