EGR1 Antibody

CAT: 0013-GTR18186641Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18186641Size:100 µg

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Description

EGR-1 antibody

Product Name Alternative

Rabbit anti-EGR-1 Antibody, EGR1, EGR 1, AT225 antibody, Early growth response 1 antibody, KROX24 antibody, Nerve growth factor-induced protein A antibody, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, ZNF225, Zinc finger protein 225, Zinc finger protein Krox-24

UniProt

P18146

Reactivity

Human, Mouse

Immunogen

This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 85-115 of Human EGR-1.

Target

EGR1

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

0.93 mg/mL

Dilution

ELISA: 1:4,000 - 1:16,000, IHC: 2 μg/ml to 20 μg/ml, WB: 1:500 - 1:3,000

Purity

This affinity purified antibody is directed against human EGR-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with EGR-1.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~58 kDa in size corresponding to EGR-1 by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_001955.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P18146

Early growth response protein 1

EGR1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P18146
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: EGR1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: EGR-1
EC number: —
Processing: —
Secondary accessions: —

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

ActivatorDNA-binding

Biological process

Biological rhythmsTranscriptionTranscription regulation

Cellular component

CytoplasmNucleus

PTM

Isopeptide bondUbl conjugation

Ligand

Metal-bindingZinc

Domain

RepeatZinc-finger

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Alternative Products

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BF0443-01	EGR1 Antibody