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SFRP1 Antibody

CAT: 0013-GTR18186612Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186612Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SFRP1 antibody
Product Name Alternative
Rabbit anti-SFRP1 antibody, SFRP-1, secreted frizzled related protein 1 antibody, FRP-1 antibody, FRP 1 antibody, FRP1 antibody, FrzA antibody, SARP 2 antibody, SARP2 antibody, Secreted Apoptosis-related Protein 2 antibody
UniProt
Q8N474
Reactivity
Human
Immunogen
SFRP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a 12 aa region of human Sfrp1 protein.
Target
SFRP1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000 - 1:15,000, IHC: 1:800, IF: Use at a concentration of 5 μg/mL, WB: 1:200-1:2,000
Purity
This affinity purified antibody is directed against human SFRP protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. Expect cross reactivity with SFRP1 from mouse, rat and bovine sources as only a single amino acid residue change is found within the immunogen sequence (91% positive by BLAST) . Cross reactivity with SFRP1 homologues from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA, western blot, and IHC. This antibody is suitable for immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Sfrp1 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IF, IHC, WB
NCBI Accession Number
56117838
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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