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CCNB1 Antibody

CAT: 0013-GTR18186608Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186608Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cyclin B1 (phospho-S126) antibody
Product Name Alternative
Rabbit anti-Cyclin B1 pS126 Antibody, Cyclin B1, CCNB1, CCNB, G2/mitotic-specific cyclin-B1
UniProt
P14635
Reactivity
Human
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 115-140 of Human Cyclin B1 protein.
Target
CCNB1
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
2.01 mg/mL
Dilution
ELISA: 1:50,000, IP: 1:100, WB: 1:100 - 1:1,000
Purity
This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption (s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Cyclin B1 from human. Cross reactivity may occur with Cyclin B1 from rat, dog and chimpanzee as only a single amino acid change was detected within the immunogen sequence from these sources (91% homology) . Partial reactivity may also occur with Cyclin B1 from mouse and hamster where two amino acids changes was noted (83% homology) . Minimal reactivity is expected with the non-phosphorylated form of the protein.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 48 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IP, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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