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IRS1 Antibody

CAT: 0013-GTR18186596Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186596Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IRS1 (phospho-S307) antibody
Product Name Alternative
Rabbit anti-IRS1 pS307 antibody, IRS-1, IRS 1, HIRS 1 antibody, HIRS-1 antibody, Insulin Receptor Substrate 1 antibody
UniProt
P35568
Reactivity
Human
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 290-320 of human IRS1 protein.
Target
IRS1
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:2,000 - 1:10,000, WB: 1:250 - 1:1,500
Purity
This affinity-purified antibody is directed against the phosphorylated form of human IRS1 protein at the pS307 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human IRS1 pS307 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human IRS1 is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with IRS1 from human, mouse, rat, dog and vervet monkey based on 100% homology with the immunizing sequence. Partial reactivity is also expected against IRS1 from pig (94%), bovine (94%) and chicken (88%) sources. Reactivity of this antibody with IRS1 from other species is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to phosphorylated IRS1 protein by western blotting in the appropriate cell lysate or extract. Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS307 of IRS1 protein.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_005535.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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