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PPARG Antibody

CAT: 0013-GTR18186586Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186586Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PPAR Gamma 2 antibody
Product Name Alternative
Rabbit anti-PPAR gamma 2 antibody, Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3, PPARG, PPAR gamma, PPARG2 antibody
UniProt
P37231
Reactivity
Mouse
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human PPAR gamma 2.
Target
PPARG
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:4,000 - 1:40,000, WB: 1:1,000 - 1:3,000
Purity
This affinity purified antibody is directed against human PPAR gamma isoform 2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human and macaque based on 100% homology for the immunogen sequence. Cross reactivity with PPARg2 protein from dog and swine is likely due to 94% homology (15/16 identities) with the protein from these sources. Cross reactivity does occur with PPARg2 from mouse. Mouse PPARg2 shows 87% homology to the immunogen. Cross reactivity with PPARg2 homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a single band approximately 55 kDa in size corresponding to PPARg2 by western blot in the appropriate tissue or cell lysate.
Tested Applications
ELISA, WB
NCBI Accession Number
20336229
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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