Products for Research Use Only
RegisterLogin

RELA Antibody

CAT: 0013-GTR18186570Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18186570Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
NFkB p65 (phospho-S276) antibody
Product Name Alternative
Rabbit anti-NFKB p65 pS276 antibody, rabbit anti-p65 antibody, rabbit anti-RelA antibody, NFKB, NFkß, NF-kB, NF-kappaB, NFkappaB, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3
UniProt
Q04206
Reactivity
Human
Immunogen
NFkB p65 (Rel A) peptide corresponding to an internal region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) .
Target
RELA
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000 - 1:25,000, WB: 1:1,000
Purity
Rabbit Anti-NFKB p65 (RelA) pS276 Antibody was prepared from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS276 human p65. Reactivity with non-phosphorylated pS267 is minimal. Cross reactivity with pS276 phosphorylated p65 from mouse, rat or other species has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-NFKB phospho specific polyclonal antibody reacts with human pS276 p65 and shows minimal reactivity when tested by western blot with non-phosphorylated p65 and minimal reactivity when tested by ELISA against the non-phosphorylated form of the immunizing peptide. For immunoblotting a 1:1,000 dilution is recommended. A 65 kDa band corresponding to human p65 is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
Tested Applications
ELISA, WB
NCBI Accession Number
223468676
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

Related Products

CatalogName
GTR18294193-01RELA polyclonal antibody
GTR18284998RELA Conjugated Antibody
GTR19061318RELA Peptide
GTR17875885Rela Rabbit Polyclonal Antibody
GTR18225773RELA/NFKB p65 Antibody
GTR18030693-01RELA Rabbit Polyclonal Antibody
GTR18683845-01RELA Rabbit Polyclonal Antibody
GTR18336143-01RELA Mouse Monoclonal Antibody
GTR18224293RELA/NFKB p65 Antibody
GTR17864590RELA Rabbit Polyclonal Antibody

Alternative Products

CatalogName
10R-1515RELA antibody

Popular Products