Products for Research Use Only

ING3 Antibody

CAT: 0013-GTR18186555Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186555Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
P47 ING3 antibody
Product Name Alternative
Goat anti-p47 ING3 antibody, p47ING3, ING-3, ING 3, Inhibitor of growth family member 3 antibody, Inhibitor of growth protein 3 antibody
UniProt
Q9NXR8
Reactivity
Human
Immunogen
This affinity purified antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 285-310 of Human ING3 protein (Inhibitor of growth family, member 3) .
Target
ING3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Concentration
1.10 mg/mL
Dilution
ELISA: 1:10,000 - 1:40,000, WB: 1:200 - 1:2,000
Purity
This is an affinity-purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human p47 ING3 protein. This sequence only shows homology to isoform 1 for ING3. The sequence is not present in isoforms 2 and 3. However, 100% homology is on record for this protein from human, dog, rat, chimpanzee and orangutan. Cross reactivity with p47 ING3 protein from mouse and chicken sources is also expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p47 ING3 proteins from other sources is not known.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA against the immunizing peptide. Specific conditions for western blotting reactivity should be optimized by the end user. Expect a band at approximately 47 kDa in size corresponding to ING3 isoform 1 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
38201655
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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