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Gdf15 Antibody

CAT: 0013-GTR18187534Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18187534Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NAG-1 antibody
Product Name Alternative
Rabbit anti-NAG-1 antibody, NAG1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor
UniProt
Q9Z0J7
Reactivity
Human, Mouse
Immunogen
This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to full-length mouse NAG-1 protein. Cross reactivity to MBP was removed via cross-adsorption chromatography.
Target
Gdf15
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.13 mg/mL
Dilution
ELISA: 1:10,000, WB: 1:1000
Purity
This product was Protein-A purified and MBP-cross-adsorbed from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with endogenous NAG-1 protein from mouse and human tissues. A BLAST analysis suggests reactivity with NAG-1 from mouse and rat based on 100% and 97% homology, respectively, and with chinese hamster based on an 92% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This Protein-A purified, MBP-cross-adsorbed NAG-1 antibody has been tested in ELISA and western blotting of mouse and human NAG-1 protein. For detection of NAG-1 in mouse serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal) specific antibodies (see Related products) . Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of approximately 14 and 28 kDa in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_035949
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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