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ORF2 Antibody

CAT: 0013-GTR18187379Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18187379Size:25 µL
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Description
L1/ORF2 antibody
Product Name Alternative
Chicken anti-L1/ORF2 Antibody, chicken anti-L1-ORF2 Antibody, L1 (LINE-1), Long INterspersed Element , retrotransposon ORF2
UniProt
O00370
Reactivity
Human, Primate
Immunogen
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH) . The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
Target
ORF2
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000, WB: 1:2,000 - 1:10,000
Purity
This IgY fraction antibody is directed against two regions within the endonuclease domain of L1 ORF2 protein. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays. A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100% homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88% homology with the immunizing sequences.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
This IgY fraction antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 149 kDa in size corresponding to L1 protein by western blotting in the appropriate cell lysate or extract.
Tested Applications
DOT, ELISA, WB
NCBI Accession Number
AAD39215.1
Host or Source
Gallus
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgY

UniProtKB · O00370

LINE-1 retrotransposable element ORF2 protein

LORF2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O00370
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
—
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
ORF2p
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

DNA recombination

Molecular function

EndonucleaseHydrolaseMultifunctional enzymeNucleaseNucleotidyltransferaseRNA-bindingRNA-directed DNA polymeraseTransferase

Ligand

MagnesiumMetal-binding

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