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UBA1 Antibody

CAT: 0013-GTR18187102Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18187102Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ubiquitin Activating Enzyme E1 antibody
Product Name Alternative
Rabbit anti-Ubiquitin Activating Enzyme E1 Antibody, A1S9 antibody, A1S9 protein antibody, A1S9T and BN75 temperature sensitivity complementing antibody, A1S9T antibody, A1ST antibody, GXP 1 antibody, rabbit anti-UBE1
UniProt
P22314
Reactivity
Human
Immunogen
Anti-Ubiquitin Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1.
Target
UBA1
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.2 mg/mL
Dilution
ELISA: 1:2,000 - 1:10,000, IHC: 2 mg/ml - 20 μg/ml, WB: 1:1,000 - 1:5,000
Purity
This antibody is directed against human Ubiquitin Activating Enzyme E1 protein. The product was protein A purified from monospecific antiserum followed by further purification to remove the GST tag. A BLAST analysis was used to suggest that this antibody would react with Ubiquitin Activating Enzyme E1 protein from human (100%) rabbit (96%), mouse (95%), rat (95%) and dog (93%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~118 kDa in size corresponding to UBE1 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
23510338
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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