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LYZ Antibody

CAT: 0013-GTR18186913Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186913Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Lysozyme antibody
Product Name Alternative
Rabbit anti-Lysozyme Antibody, 1 4 beta n acetylmuramidase c antibody, EC 3.2.1.17 antibody, LYZ antibody, Lysozyme C antibody, Lysozyme C precursor antibody, LZM antibody
UniProt
P00698
Reactivity
Gallus
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme.
Target
LYZ
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
1.0 mg/ml
Dilution
ELISA: 1:25,000, IHC: 1:500 - 1:2,000, IP: 1:100, WB: 1:500 - 1:2,000
Purity
This purified antibody is directed against lysozyme from hen egg white protein. This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process, which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lysozyme (Hen Egg White) . A BLAST analysis was used to suggest that this antibody would react with all forms of lysozyme from chicken, including precursor as well as A and B chains. Chains designated as M, L, F, C, Y and D also show 100% sequence homology. Lysozyme from quail and pheasant are also reported to be 100% identical with HEW lysozyme. Cross reactivity against lysozyme from other sources may occur but has not been specifically determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Lysozyme Hen Egg White purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~14 kDa in size corresponding to lysozyme by western blotting in the appropriate cell lysate or extract.
Tested Applications
DOT, ELISA, IHC, IP, WB
NCBI Accession Number
NP_990612.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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