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GDF15 Antibody

CAT: 0013-GTR18186780Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186780Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NAG-1 antibody
Product Name Alternative
Mouse anti-NAG1 Antibody, NAG-1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor
UniProt
Q99988
Reactivity
Human
Immunogen
This Protein A purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH.
Target
GDF15
Clonality
Monoclonal
Clone
23G10.E4.F8
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:200,000, WB: 1:1,000
Purity
This product was purified from concentrated tissue culture supernatant Protein A chromatography. This antibody specifically reacts with the amino terminal end of human NAG-1 protein from human tissues. A BLAST analysis was used to suggest partial reactivity with NAG-1 from chimpanzee and macaque based on a 92% homology. Multimeric forms of NAG-1 may be detected. Cross-reactivity with NAG-1 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This Protein A purified anti-NAG1 antibody has been tested by ELISA and western blotting for human NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 C-terminal specific antibodies. This antibody is useful in dual antibody immunometric assays (EIA) . Specific conditions for reactivity should be optimized by the end user. Expect bands in western blots of approximately 14 kDa in size corresponding to NAG-1 monomer using the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
Q99988.3
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG1

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