1a Antibody

CAT: 0013-GTR18186724Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18186724Size:100 µg

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Description

Hepatitis Virus A59 Nonstructural Protein 9 antibody

Product Name Alternative

Mouse anti-Hepatitis Virus A59 Nonstructural Protein 9 Antibody, mouse anti-MHV-A59 nsp9 protein antibody

UniProt

P0C6V0

Reactivity

Mouse

Immunogen

This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.

Target

Clonality

Monoclonal

Clone

2C6.H1

Conjugation

Unconjugated

Concentration

2.01 mg/mL

Dilution

IF: 1:1,000, WB: 1:1,000

Purity

This antibody is directed against the MHV-A59 nsp9 protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography. No cross reactivity occurs with SARS CoV nsp9. Cross reactivity with homologues from other sources has not been tested.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3% PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.

Tested Applications

IF, WB

NCBI Accession Number

25121567

Host or Source

Mouse

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG2b

UniProtKB · P0C6V0

Replicase polyprotein 1a

R1A_CVMA5 · Murine coronavirus (strain A59)

View on UniProt ↗

Primary accession: P0C6V0
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: —
Protein existence: 1: Evidence at protein level
Organism: Murine coronavirus (strain A59) (MHV-A59)
Taxonomy ID: 11142
Alternative names: pp1a
EC number: —
Processing: —
Secondary accessions: P16342

Protein keywords

Technical term

3D-structureReference proteome

Biological process

Activation of host autophagy by virusDecay of host mRNAs by virusEukaryotic host gene expression shutoff by virusEukaryotic host translation shutoff by virusHost gene expression shutoff by virusHost mRNA suppression by virusHost-virus interactionInhibition of host innate immune response by virusInhibition of host interferon signaling pathway by virusInhibition of host IRF3 by virusInhibition of host ISG15 by virusInhibition of host RLR pathway by virusInterferon antiviral system evasionModulation of host ubiquitin pathway by viral deubiquitinaseModulation of host ubiquitin pathway by virusUbl conjugation pathwayViral immunoevasion

PTM

Disulfide bond

Molecular function

EndonucleaseHydrolaseMethyltransferaseNucleaseProteaseRNA-bindingThiol proteaseTransferase

Cellular component

Host cytoplasmHost membraneMembrane

Ligand

Metal-bindingZinc

Domain

RepeatTransmembraneTransmembrane helixZinc-finger

Coding sequence diversity

Ribosomal frameshifting

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