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1a Antibody

CAT: 0013-GTR18186724Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186724Size:100 µg
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Description
Hepatitis Virus A59 Nonstructural Protein 9 antibody
Product Name Alternative
Mouse anti-Hepatitis Virus A59 Nonstructural Protein 9 Antibody, mouse anti-MHV-A59 nsp9 protein antibody
UniProt
P0C6V0
Reactivity
Mouse
Immunogen
This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.
Target
1a
Clonality
Monoclonal
Clone
2C6.H1
Conjugation
Unconjugated
Concentration
2.01 mg/mL
Dilution
IF: 1:1,000, WB: 1:1,000
Purity
This antibody is directed against the MHV-A59 nsp9 protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography. No cross reactivity occurs with SARS CoV nsp9. Cross reactivity with homologues from other sources has not been tested.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3% PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.
Tested Applications
IF, WB
NCBI Accession Number
25121567
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG2b

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