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AKT1 Antibody

CAT: 0013-GTR18186768Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18186768Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Akt (phospho-S473) antibody
Product Name Alternative
Mouse anti-AKT pS473 Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
UniProt
P31749
Reactivity
Human, Monkey, Mouse, Rat
Immunogen
Anti-AKT pS473 antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein, followed by hybridoma development
Target
AKT1
Clonality
Monoclonal
Clone
17F6.B11
Conjugation
Unconjugated
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Dilution
ELISA: 1:20,000, IHC: 20 μg/mL, IF: 1:500 - 1:3,000, WB: 1:500 - 1:3,000
Purity
Anti-AKT pS473 (MOUSE) Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
This monoclonal antibody is tested in ELISA, immunofluorescence, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.
Tested Applications
ELISA, IF, IHC, Multiplex Assay, WB
NCBI Accession Number
62241011
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG1

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