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CDK1 Antibody

CAT: 0013-GTR18186545Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186545Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cdc2 P34 antibody
Product Name Alternative
Mouse anti-Cdc2 antibody, mouse anti-p34 antibody, Cell Division Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, p34 Cdk1 antibody, p34 protein kinase antibody, CDC28A, CDKN1, P34CDC2
UniProt
P06493
Reactivity
Human
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.
Target
CDK1
Clonality
Monoclonal
Clone
POH-1
Conjugation
Unconjugated
Field of Research
Cell Biology
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:1,000
Purity
This protein A purified mouse monoclonal antibody reacts specifically with p34 cdc2 in human tissues and cell lines. This antibody is not cross reactive with other cyclin dependent kinases. Cross reactivity with p34 cdc2 from other sources, especially mouse and rat will occur. This reagent has broad interspecies reactivity.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-p34 cdc2 has been tested by western blot and is suitable for immunohistochemistry, immunoprecipitation (as active kinase), and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products. HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting. LEP fibroblast cell lysate is suggested as a negative control. Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1% saponin treatment or other permeabilization method.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
NP_001307847.1
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Isotype
IgG2a

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