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HIF1A Antibody

CAT: 0013-GTR18187383Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18187383Size:100 µL
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Description
HIF1A antibody
Product Name Alternative
Rabbit anti-Hif-1 alpha hydroxy P564 Antibody, Hypoxia-inducible factor 1-alpha antibody, ARNT-interacting protein antibody, Member of PAS protein 1 antibody, Basic-helix-loop-helix-PAS protein MOP1 antibody, PAS domain-containing protein 8 antibody, Class E basic helix-loop-helix protein 78 antibody
UniProt
Q16665
Reactivity
Human
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding the P564 of human HIF-1a.
Target
HIF1A
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Concentration
65 mg/mL
Dilution
ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000
Purity
This antibody is directed against human HIF-1a hydroxyP564 and is specific for the hydroxylated form of the protein. Minimal reactivity occurs with the non-hydroxylated form of the protein. This antibody is specific for HIF-1a hydroxylated at P564. Minimal cross-reactivity occurs with non-hydroxylated HIF-1a. A BLAST analysis was used to suggest cross-reactivity with HIF-1a from human, monkey, mouse, rat, dog, bovine and Xenopus sources based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 110 kDa in size corresponding to HIF-1a hydroxyl P564 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
4504385
Host or Source
Rabbit
Preservative
0.01% (w/v) Sodium Azide
Isotype
Antiserum

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