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NOTCH2 Antibody

CAT: 0013-GTR18187345Size: 200 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18187345Size:200 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NOTCH2 antibody
Product Name Alternative
Rabbit anti-Notch 2 antibody, AGS 2 antibody, AGS2 antibody, hN2 antibody, N2 antibody, Neurogenic locus notch homolog protein 2 antibody, Notch 2 intracellular domain antibody, Notch homolog 2 antibody
UniProt
Q04721
Reactivity
Human
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid residues 2390-2415 of human Notch 2 (the total protein is 2471 aa) . A residue of cysteine was added to the amino terminal end to facilitate coupling.
Target
NOTCH2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Concentration
75 mg/mL
Dilution
ELISA: 1:30,000 - 1:90,000, IHC: 1:200-1:800, WB: 1:400 - 1:2,000
Purity
This antiserum is directed against human NOTCH 2. The peptide sequence shows 100% alignment with human, dog and chimpanzee sequence. Only one (1) amino acid difference is found in mouse and this change is non-conservative. Based on the sequence we expect this antibody to react as well with rat and mouse NOTCH 2. No specific information is available for other reactivities.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This antibody has been tested for use in ELISA and western blot. For western blot experiments, one can expect a band of ≈110 kDa in size corresponding to active Notch 2 in the appropriate cell lysate. A 1:500 dilution was effective for staining FFPE human kidney tissue by Immunohistochemistry (IHC) . Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
24041035
Host or Source
Rabbit
Preservative
0.1% (w/v) Sodium Azide
Isotype
Antiserum

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