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Digoxigenin Antibody

CAT: 0013-GTR18187233Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18187233Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant DIG (DIG44) antibody
Product Name Alternative
Anti-Digoxigenin, Digoxigenin (DIG), 1672-46-4, Lanadigenin, Digoxigenine, UNII-NQ1SX9LNAU, DIG, CHEBI:42098, HSDB 7108, NQ1SX9LNAU, EINECS 216-806-2, BRN 0096479, 4- (3,12,14-Trihydroxy-10,13-Dimethyl-Hexadecahydro-Cyclopenta[a]Phenanthren-17-Yl) -5h-Furan-2-One
Reactivity
Bacteria
Target
Digoxigenin
Clonality
Recombinant
Clone
DIG44
Conjugation
Unconjugated
Source
E. coli
Concentration
1.0 mg/mL
Dilution
ELISA: 1:1000, WB: 1:1000
Purity
Anti-DIG (VHH) Antibody is a recombinant antibody. The clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture. Expect reactivity against DIG-labeled probes and proteins.
Form
Liquid (sterile filtered)
Storage Conditions
Store at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-DIG is a his-tagged monoclonal recombinant antibody designed to detect digoxigenin and its conjugates. This antibody has been tested by ELISA, SDS-PAGE, and western blot and is intended for use in immunological assays including ELISA, western blotting, immunofluorescence and fluorescence activated cell sorting (FACS) . Fluorochrome conjugated anti-DIG can be used for detection of sensitive non-radioactive in situ hybridization probes. The antibody can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection of DIG-conjugated molecules by immunoblotting or immunofluorescence. Secondary detection can be achieved using conjugated anti-His tag or anti-VHH antibodies. Optimal titers for applications should be confirmed by the end user.
Tested Applications
ELISA, WB
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 20% (v/v) Glycerol; Buffer: 1X PBS, pH 7.4

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