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ACTB Antibody (HRP)

CAT: 0013-GTR18187160Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18187160Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Beta Actin antibody (Peroxidase)
Product Name Alternative
Rabbit anti-beta Actin Antibody HRP conjugation, peroxidase conjugated rabbit anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody
UniProt
P60709
Reactivity
Bovine, Goat, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rat, Sheep
Immunogen
Beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 350-375 of Human beta Actin.
Target
ACTB
Clonality
Polyclonal
Conjugation
HRP
Field of Research
Cancer Biology
Concentration
1.0 mg/mL
Dilution
ELISA: 1:20,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
Purity
Beta Actin Antibody Peroxidase Conjugated antibody is directed against human beta Actin protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Beta Actin Antibody Peroxidase Conjugated antibody has been tested for use in ELISA and is suitable in immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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