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CDC16 Antibody

CAT: 0013-GTR18186667Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186667Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
APC6 (phospho-T580) antibody
Product Name Alternative
Rabbit anti-APC6 pT580 Antibody, APC 6, APC-6, Anaphase promoting complex subunit 6 antibody, CDC16Hs antibody, Cell division cycle 16 homolog antibody, Cell division cycle protein 16 homolog antibody, Cyclosome subunit 6 antibody
UniProt
Q13042
Reactivity
Human
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding pT580 of Human Apc6 protein.
Target
CDC16
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
0.97 mg/mL
Dilution
ELISA: 1:1,000 - 1:10,000, WB: 1:500 - 1:3,000
Purity
This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption (s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human APC6 protein at the pT580 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with APC6 from human. No cross reactivity is expected against homologues from other sources. This antibody is expected to react with all isoforms of this protein. Minimal reactivity is expected with the non-phosphorylated form of the protein.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 72 kDa, 71.5 kDa or 65.9 kDa in size corresponding to isoforms of APC6 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
37537763
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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