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BMI1 Antibody

CAT: 0013-GTR18186568Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18186568Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
BMI1 antibody
Product Name Alternative
Goat anti-Bmi-1 antibody, Bmi1, B lymphoma Mo MLV insertion region antibody, Bmi 1 antibody, MGC12685 antibody, Murine leukemia viral (bmi 1) oncogene homolog antibody, Oncogene BMI 1 antibody, PCGF 4 antibody
UniProt
P35226
Reactivity
Human
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 252-264 of human Bmi1 protein.
Target
BMI1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Concentration
0.91 mg/mL
Dilution
ELISA: 1:5,000 - 1:30,000, IF: 1:200, WB: 1:500 - 1:3,000
Purity
This affinity purified antibody is directed against human Bmi1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Bmi1 protein from human, rat, orangutan, dog, chimpanzee, bovine and cat based on 100% homology to the immunogen sequence. This sequence shows significant homology to homologues from Xenopus and chicken sources. Reactivity against homologues from other sources is not known.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA, immunofluorescence, and western blot. A 1:200 dilution is reported to be effective for Immunofluorescence against Methanol fixed cells. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Bmi1 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IF, Multiplex Assay, WB
NCBI Accession Number
27883842
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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