Products for Research Use Only

Lep Antibody

CAT: 0013-GTR18187512Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18187512Size:1 mg
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Description
Leptin antibody
Product Name Alternative
Rabbit anti-leptin antibody, LEP antibody, Leptin Murine Obesity Homolog antibody, Leptin Precursor Obesity Factor antibody, OB antibody, Obese Protein antibody
UniProt
P41160
Reactivity
Human, Mouse
Immunogen
Anti-Leptin antibody was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli.
Target
Lep
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000
Purity
Anti-Leptin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. The antibody will recognize leptin from mouse and human. Reactivity with leptin from other sources is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This IgG fraction of anti-Mouse Leptin was tested for use in ELISA and immunoblotting. Reactivity in other immunoassays is unknown. The antibody will recognize recombinant and native 16 kDa leptin from mouse and human.
Tested Applications
ELISA, WB
NCBI Accession Number
P41160.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG