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TEV Protease Antibody

CAT: 0013-GTR18187268Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18187268Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TEV Protease antibody
Product Name Alternative
Rabbit anti-TEV Protease Antibody, Tobacco Etch Virus, tobacco etch potyvirus, TEV, TEV Protease
UniProt
P04517
Reactivity
Other
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant MBP-and-poly-His-tagged auto inactivation-resistant mutant TEV Protease.
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Protein Biochemistry
Concentration
1.01 mg/mL
Dilution
ELISA: 1:1,500-1:27,000, WB: 1:500
Purity
This product was protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. Assay by western blot showed no reactivity to MBP.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This protein A purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 27 kDa in size corresponding to TEV by western blotting in the appropriate cell lysate or extract. Anti-TEV protease may be useful to detect residual TEV protease in preparations of recombinant proteins in which that protease may interfere with downstream manipulations.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_734212.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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