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Mouse IgG2b isotype Control Antibody

CAT: 0013-GTR18184672Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184672Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse IgG2b isotype Control Antibody
Product Name Alternative
MOUSE IgG2b subclass isotype control, MOUSE IgG2b isotype control
Clone
M2B
Conjugation
Unconjugated
Source
Mouse
Field of Research
Protein Biochemistry
Concentration
1.0mg/mL
Dilution
FC: 1:1000-1:5000
Purity
Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Extensive cross-adsorption was performed to remove any unwanted subclasses. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgA, IgM. Confirmed by SDS-PAGE.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Mouse IgG2b isotype control has been tested by SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Tested Applications
FC, SDS-PAGE
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG2b

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