COL3A1 Antibody (Biotin)

CAT: 0013-GTR18184589Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18184589Size:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Collagen Type III antibody (Biotin)

Product Name Alternative

Rabbit anti-Collagen Type III antibody biotin conjugation, biotin conjugated rabbit anti-Collagen Type III antibody, Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody, COL3A1, Collagen alpha-1 (III) chain

UniProt

P02461

Reactivity

Bovine, Human

Immunogen

Collagen Type III from human and bovine placenta

Target

COL3A1

Clonality

Polyclonal

Conjugation

Biotin

Concentration

1.0 mg/ml

Dilution

ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, IP: 1:100, WB: 1:1,000 - 1:5,000

Purity

This product has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has expected cross-reactivity with Type I and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.

Form

Lyophilized

Storage Conditions

Store vial at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.

Tested Applications

ELISA, IHC, IP, WB

NCBI Accession Number

NP_000081.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P02461

Collagen alpha-1(III) chain

CO3A1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P02461
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: COL3A1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: D2JYH5, D3DPH4, P78429, Q15112, Q16403, Q53S91, Q541P8, Q6LDB3, Q6LDJ2, Q6LDJ3, Q7KZ56, Q8N6U4, Q9UC88, Q9UC89, Q9UC90, Q9UC91, R4N3C5, V9GZI1

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Disease

Aortic aneurysmDisease variantEhlers-Danlos syndrome

Ligand

CalciumMetal-binding

Domain

CollagenRepeatSignal

PTM

Disulfide bondGlycoproteinHydroxylation

Cellular component

Extracellular matrixSecreted

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