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CDKN1B Antibody

CAT: 0013-GTR18184549Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184549Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
P27 kip1 pS140 antibody
Product Name Alternative
Rabbit anti-p27 kip1 pS140 antibody, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Kip-1, KIP1, CDKN1B
UniProt
P46527
Reactivity
Human
Immunogen
Anti-p27 kip1 pS140 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to S140 residue of human p27 kip1 protein.
Target
CDKN1B
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Concentration
1.23 mg/mL
Dilution
ELISA: 1:10,000 - 1:40,000, WB: 1:500 - 1:4,000
Purity
Anti-p27 kip1 pS140 is directed against the phosphorylated form of human p27 kip1 protein at the S140 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. This antibody will specifically react with a p27kip1 protein from human tissue. Reactivity against p27 from other species is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-p27kip1 pS140 antibody is tested for use in ELISA, immunoprecipitation, co-immunoprecipitation, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~27 kDa in size corresponding to p27 by western blotting in the appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human p27. Optimal titers for other applications should be determined by the researcher.
Tested Applications
ELISA, WB
NCBI Accession Number
4757962
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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