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HTT Antibody

CAT: 0013-GTR18184791Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184791Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Huntington antibody
Product Name Alternative
Rabbit anti-Huntington antibody, rabbit anti-Huntingtin antibody, HD protein, HTT, Huntington Disease Protein, Huntington disease, IT15, LOMARS
UniProt
P42858
Reactivity
Human, Mouse, Rat
Immunogen
Huntington affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the near N-terminus of human Huntington disease protein.
Target
HTT
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Concentration
1.1 mg/ml
Dilution
ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF: 1:100-1:500, WB: 0.1 μg/ml
Purity
Anti-Huntingtin was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HTT. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, and Rat based on 100% sequence homology. Cross-reactivity with HTT from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Huntingtin antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~350 kDa corresponding to the appropriate cell lysate or extract.
Tested Applications
ELISA, IF, IHC, WB
NCBI Accession Number
NP_002102.4
Host or Source
Rabbit
Preservative
Preservative: None. Stabilizer: 50% (v/v) Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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