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H2AFX Antibody

CAT: 0013-GTR18184602Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18184602Size:25 µL
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Description
H2AX pS139 antibody
Product Name Alternative
Rabbit anti-H2AX pS139 antibody, rabbit anti-Histone H2AX pS139 antibody, H2AFX antibody, H2a/x, H2A.x, H2AX, histone H2A, gamma-H2AX, H2A histone family member X antibody
UniProt
P16104
Reactivity
Human
Immunogen
Anti-H2AX pS139 purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the C-terminal region containing serine 139 of human H2AX protein.
Target
H2AFX
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000, WB: 1:1,000
Purity
H2AX pS139 is directed against the phosphorylated form of human H2AX protein at the pS139 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity with non-phosphorylated human H2AX is minimal by ELISA and western blot. A BLAST analysis was used to suggest 100% cross reactivity with H2AX from human based on the sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-H2AX pS139 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in size corresponding to phosphorylated H2AX protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_002096.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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