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SPRY2 Antibody

CAT: 0013-GTR18184538Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18184538Size:25 µL
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Description
Sprouty-2 antibody
Product Name Alternative
Rabbit anti-Sprouty2 antibody, Rabbit anti-Sprouty-2 antibody, Sprouty 2, SPRY2, SPRY 2, Spry-2, Protein sprouty homolog 2
UniProt
O43597
Reactivity
Human
Immunogen
Anti-Sprouty-2 affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with peptide corresponding to an internal region of human sprouty-2.
Target
SPRY2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Concentration
1.04 mg/mL
Dilution
ELISA: 1:5000-1:50000, IHC: 10μg/ml, IF: 1:250-1:500, WB: 1:500-1:5,000
Purity
Anti-Sprouty-2 was affinity purified from monospecific antiserum by immunoaffinity chromatography. Sprouty-2 antibody reacts with human Sprouty 2 protein. A BLAST analysis was used to suggest reactivity with Sprouty 2 from human, monkey, and orangutan based on a 100% homology with the immunizing sequence. Cross-reactivity with Sprouty2 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Sprouty-2 antibody has been tested by ELISA, Immunofluorescence, Immunohistochemistry, and is suitable for western blotting. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, IF, IHC, Multiplex Assay, WB
NCBI Accession Number
AAC04258.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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