RPS6KA1 Antibody

CAT: 0013-GTR18184531Size: 25 µLDry Ice: NoHazardous: No

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CAT#:0013-GTR18184531Size:25 µL

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Description

P90 RSK1 antibody

Product Name Alternative

Rabbit anti-p90 RSK1 antibody, rabbit anti-RSK1 antibody, Ribosomal S6 Kinase 1, RSK-1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, MAPK-activated protein kinase 1a, p90-RSK 1, p90S6K, MAPKAP kinase 1a, MAPKAPK-1a, Ribosomal S6 kinase 1, RSK 1, RPS6KA1, MAPKAPK1A

UniProt

Q15418

Reactivity

Human

Immunogen

Anti-p90 RSK1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.

Target

RPS6KA1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Concentration

1.0 mg/ml

Dilution

ELISA: 1:5000 - 1:50,000, WB: 1μg/mL

Purity

Anti-p90 RSK1 was affinity purified from monospecific antiserum by immunoaffinity chromatography using phosphorylated peptide coupled to agarose beads followed by solid phase adsorption against non phosphorylated peptide. This antibody is specific for human p90 RSK1 protein. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100% homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-p90 RSK1 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 90 kDa in size corresponding to p90 RSK1 protein is expected in the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_001006666.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · Q15418

Ribosomal protein S6 kinase alpha-1

KS6A1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q15418
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: RPS6KA1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: S6K-alpha-1
EC number: 2.7.11.1
Processing: —
Secondary accessions: A6NGG4, A8K9K7, B2RDY8, B7Z5J0, E9PRI4, Q5SVM5, Q5SVM8, Q5SVM9, Q96C05, Q9BQK2

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingMagnesiumMetal-bindingNucleotide-binding

Biological process

Cell cycleHost-virus interactionStress response

Cellular component

CytoplasmNucleus

Molecular function

KinaseSerine/threonine-protein kinaseTransferase

PTM

Phosphoprotein

Domain

Repeat

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