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BrdU Antibody

CAT: 0013-GTR18184516Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18184516Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
BrdU antibody
Product Name Alternative
Rabbit anti-BrdU antibody, bromodeoxyuridine, 5-bromo-2'-deoxyuridine
Reactivity
Other
Immunogen
Anti-BrdU affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with BromodeoxyUridine-KLH.
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.1 mg/mL
Dilution
ELISA: 1:2000 - 1:10,000, FC: 1:50-1:100, IHC: 1:100-1:500, IF: 1:500
Purity
BrdU Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Form
Liquid (sterile filtered)
Storage Conditions
Store BrdU Antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-BrdU Antibody has been tested by ELISA, slot blot, IHC, and is suitable for immunofluorescence microscopy and flow cytometry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incorporated BrdU thymidine analog from replicated cells
Tested Applications
DOT, ELISA, FC, IF, IHC
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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