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RPS6KA1 Antibody

CAT: 0013-GTR18184503Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184503Size:100 µg
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Description
P90 RSK1 (phospho-S732) antibody
Product Name Alternative
Rabbit anti-p90 RSK1 pS732 antibody, rabbit anti-RSK1 pS732 antibody, Ribosomal S6 Kinase 1, RSK-1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, MAPK-activated protein kinase 1a, p90-RSK 1, p90S6K, MAPKAP kinase 1a, MAPKAPK-1a, Ribosomal S6 kinase 1, RSK 1, RPS6KA1, MAPKAPK1A
UniProt
Q15418
Reactivity
Human
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.
Target
RPS6KA1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Concentration
1.0mg/mL
Dilution
ELISA: 1:5000-1:50,000, WB: 1:1000-1:3000
Purity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phosphorylated peptide coupled to agarose beads followed by solid phase adsorption against non phosphorylated peptide. This antibody is specific for human p90 RSK1 protein phosphorylated at S732. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100% homology with the immunizing sequence. Partially cross reactivity may occur against opossum and chicken based on > 90% homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity-purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 90 kDa in size corresponding to p90 RSK1 pS732 protein is expected in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_001006666.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · Q15418

Ribosomal protein S6 kinase alpha-1

KS6A1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q15418
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
RPS6KA1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
S6K-alpha-1
EC number
2.7.11.1
Processing
—
Secondary accessions
A6NGG4, A8K9K7, B2RDY8, B7Z5J0, E9PRI4, Q5SVM5, Q5SVM8, Q5SVM9, Q96C05, Q9BQK2
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingMagnesiumMetal-bindingNucleotide-binding

Biological process

Cell cycleHost-virus interactionStress response

Cellular component

CytoplasmNucleus

Molecular function

KinaseSerine/threonine-protein kinaseTransferase

PTM

Phosphoprotein

Domain

Repeat

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