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HDAC1 Antibody

CAT: 0013-GTR18184459Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18184459Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HDAC-1 antibody
Product Name Alternative
Rabbit anti-HDAC-1 antibody, HDAC1, HDAC 1, HD 1 antibody, histone deacetylase 1 antibody, Histone deacetylase-1, HD1 antibody, RPD3L1, reduced potassium dependency yeast homolog like 1 antibody
UniProt
Q13547
Reactivity
Human
Immunogen
Anti-HDAC-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 450-482 of Human HDAC-1.
Target
HDAC1
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, IF: 10ug / ml, WB: 1:1,000 - 1:5,000
Purity
Anti-HDAC-1 antibody is directed against human HDAC-1 protein. HDAC-1 antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and chimpanzee sources based on 100% homology for the immunogen sequence. Cross reactivity may occur with HDAC-1 from bovine (82% homology) and chicken (80% homology) sources. Cross reactivity with HDAC-1 homologues from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-HDAC-1 Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Specific nuclear staining is observed by IHC. Expect bands at 65 kDa in size corresponding to HDAC-1 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IF, IHC, Multiplex Assay, WB
NCBI Accession Number
13128860
Host or Source
Rabbit
Preservative
Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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